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Evaluation of the acute toxicity, phytochemical constituents and anti - ulcer properties of methanolic leaf extract of Annona muricata in mice

Valentine Uneojo Omoja, Thelma Ebele Ihedioha, Gabriel Ifeanyi Eke, Iheanyi K Peter-Ajuzie, Samuel Ekere Okezie.

Cited by (1)

Abstract
This study investigated the acute toxicity, phytochemical constituents and anti - ulcer properties of methanolic leaf extract of Annona muricata in mice. The anti - ulcer activity was evaluated using absolute ethanol-induced ulcer and aspirin-induced ulcer models in mice. An LD50 of 354.8 ± 8 mg/kg body weight, bw of the extract was obtained on oral administration. Investigation of the phytochemical constituents of the plant extract revealed the presence of saponins, alkaloids and traces of tannins. All doses of the extract (50, 75 and 100 mg/kg) used for the study significantly reduced (p<0.05) the mean number of ulcers in both ulcer models when compared to the untreated group A (10 ml/kg distil water). Optimum antiulcer activity of the extract against absolute ethanol-induced ulcer was noted at 50 mg/kg bw. At this 50 mg/kg, the mean number of ulcers and mean ulcer index of the extract was significantly lower (p<0.05) than that of Cimetidine at 100 mg/kg (3.60 ± 0.51: 5.00 ± 0.32; 1.5±0.05: 0.98±0.03), the treated control group whereas the protective index of the extract was higher than that of cimetidine (50.51 %: 24.24 %). The results obtained from this study strongly suggest that methanolic leaf extract of Annona muricata can be effectively used for the treatment of ulcer in low doses and can provide better therapeutic effect than cimetidine if used in ulcers caused by alcoholism and related agents.

Key words: Acute, anti - ulcer, Annona muricata, cimetidine


 
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This Article Cited By the following articles

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How to Cite this Article
Pubmed Style

Omoja VU, Ihedioha TE, Eke GI, Peter-Ajuzie IK, Okezie SE. Evaluation of the acute toxicity, phytochemical constituents and anti - ulcer properties of methanolic leaf extract of Annona muricata in mice. J Complement Med Res (Formerly J Intercult Ethnopharmacol). 2014; 3(1): 37-43. doi:10.5455/jice.20140111103203


Web Style

Omoja VU, Ihedioha TE, Eke GI, Peter-Ajuzie IK, Okezie SE. Evaluation of the acute toxicity, phytochemical constituents and anti - ulcer properties of methanolic leaf extract of Annona muricata in mice. http://www.jicep.com/?mno=47850 [Access: February 22, 2018]. doi:10.5455/jice.20140111103203


AMA (American Medical Association) Style

Omoja VU, Ihedioha TE, Eke GI, Peter-Ajuzie IK, Okezie SE. Evaluation of the acute toxicity, phytochemical constituents and anti - ulcer properties of methanolic leaf extract of Annona muricata in mice. J Complement Med Res (Formerly J Intercult Ethnopharmacol). 2014; 3(1): 37-43. doi:10.5455/jice.20140111103203



Vancouver/ICMJE Style

Omoja VU, Ihedioha TE, Eke GI, Peter-Ajuzie IK, Okezie SE. Evaluation of the acute toxicity, phytochemical constituents and anti - ulcer properties of methanolic leaf extract of Annona muricata in mice. J Complement Med Res (Formerly J Intercult Ethnopharmacol). (2014), [cited February 22, 2018]; 3(1): 37-43. doi:10.5455/jice.20140111103203



Harvard Style

Omoja, V. U., Ihedioha, T. E., Eke, G. I., Peter-Ajuzie, I. K. & Okezie, S. E. (2014) Evaluation of the acute toxicity, phytochemical constituents and anti - ulcer properties of methanolic leaf extract of Annona muricata in mice. J Complement Med Res (Formerly J Intercult Ethnopharmacol), 3 (1), 37-43. doi:10.5455/jice.20140111103203



Turabian Style

Omoja, Valentine Uneojo, Thelma Ebele Ihedioha, Gabriel Ifeanyi Eke, Iheanyi K Peter-Ajuzie, and Samuel Ekere Okezie. 2014. Evaluation of the acute toxicity, phytochemical constituents and anti - ulcer properties of methanolic leaf extract of Annona muricata in mice. Journal of Complementary Medicine Research (Formerly known as Journal of Intercultural Ethnopharmacology (2012-2017)), 3 (1), 37-43. doi:10.5455/jice.20140111103203



Chicago Style

Omoja, Valentine Uneojo, Thelma Ebele Ihedioha, Gabriel Ifeanyi Eke, Iheanyi K Peter-Ajuzie, and Samuel Ekere Okezie. "Evaluation of the acute toxicity, phytochemical constituents and anti - ulcer properties of methanolic leaf extract of Annona muricata in mice." Journal of Complementary Medicine Research (Formerly known as Journal of Intercultural Ethnopharmacology (2012-2017)) 3 (2014), 37-43. doi:10.5455/jice.20140111103203



MLA (The Modern Language Association) Style

Omoja, Valentine Uneojo, Thelma Ebele Ihedioha, Gabriel Ifeanyi Eke, Iheanyi K Peter-Ajuzie, and Samuel Ekere Okezie. "Evaluation of the acute toxicity, phytochemical constituents and anti - ulcer properties of methanolic leaf extract of Annona muricata in mice." Journal of Complementary Medicine Research (Formerly known as Journal of Intercultural Ethnopharmacology (2012-2017)) 3.1 (2014), 37-43. Print. doi:10.5455/jice.20140111103203



APA (American Psychological Association) Style

Omoja, V. U., Ihedioha, T. E., Eke, G. I., Peter-Ajuzie, I. K. & Okezie, S. E. (2014) Evaluation of the acute toxicity, phytochemical constituents and anti - ulcer properties of methanolic leaf extract of Annona muricata in mice. Journal of Complementary Medicine Research (Formerly known as Journal of Intercultural Ethnopharmacology (2012-2017)), 3 (1), 37-43. doi:10.5455/jice.20140111103203





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