eJManager
 
E-ISSN 2146-8397
 

Original Research Open Access


Tricyclic sesquiterpene copaene prevents H2O2-induced neurotoxicity

Hasan Turkez, Basak Togar, Abdulgani Tatar.

Cited by (2)

Abstract
Aim: Copaene (COP), a tricyclic sesquiterpene, is present in several essential oils of medicinal and aromatic plants and has antioxidant and anticarcinogenic features. But, very little information is known about the effects of COP on oxidative stress induced neurotoxicity. Method: We used hydrogen peroxide (H2O2) exposure for 6 h to model oxidative stress. Therefore, this experimental design allowed us to explore the neuroprotective potential of COP in H2O2-induced toxicity in rat cerebral cortex cell cultures for the first time. For this purpose, methyl thiazolyl tetrazolium (MTT) and lactate dehydrogenase (LDH) release assays were carried out to evaluate cytotoxicity. Total antioxidant capacity (TAC) and total oxidative stress (TOS) parameters were used to evaluate oxidative changes. In addition to determining of 8-hydroxy-2-deoxyguanosine (8-OH-dG) levels, the single cell gel electrophoresis (SCGE or comet assay) was also performed for measuring the resistance of neuronal DNA to H2O2-induced challenge.
Result: The results of this study showed that survival and TAC levels of the cells decreased, while TOS, 8-OH-dG levels and the mean values of the total scores of cells showing DNA damage increased in the H2O2 alone treated cultures. But pre-treatment of COP suppressed the cytotoxicity, genotoxicity and oxidative stress which were increased by H2O2. Conclusion: It is proposed that COP as a natural product with an antioxidant capacity in mitigating oxidative injuries in the field of neurodegenerative diseases.

Key words: Copaene, neuroprotection, H2O2, primary neuron, DNA damage, oxidative stress, in vitro


 
ARTICLE TOOLS
Abstract
PDF Fulltext
Print this article Print this Article
How to cite this articleHow to cite this article
Export to
Export to
Related Records
 Articles by Hasan Turkez
Articles by Basak Togar
Articles by Abdulgani Tatar
on Google
on Google Scholar
Article Statistics
 Viewed: 2810
Downloaded: 596
Cited: 2

REFERENCES
1. Joseph B, Martin MD. Molecular basis of the neurodegenerative disorders. N Engl J Med. 1999; 340: 1970-1980 [DOI via Crossref]    [Pubmed]   
2. Calabrese V, Lodi R, Tonon C, D'Agata V, Sapienza M, Scapagnini G, et al. Oxidative stress, mitochondrial dysfunction and cellular stress response in Friedreich's ataxia. J Neurol Sci. 2005; 233: 145-62. [DOI via Crossref]    [Pubmed]   
3. Si CL, Shen T, Jiang YY, Wu L, Yu GJ, Ren XD, et al. Antioxidant properties and neuroprotective effects of isocampneoside II on hydrogen peroxide-induced oxidative injury in PC12 cells. Food Chem Toxicol. 2013; 59: 145-152. [DOI via Crossref]    [Pubmed]   
4. Turkez H. The role of ascorbic acid on titanium dioxide-induced genetic damage assessed by the comet assay and cytogenetic tests. Exp Toxicol Pathol. 2011; 63: 453-457. [DOI via Crossref]    [Pubmed]   
5. Farah R, Khamisy-Farah R, Amit T, Youdim MB, Arraf Z. Lithium's gene expression profile, relevance to neuroprotection A cDNA microarray study. Cell Mol Neurobiol. 2013; 33:411-420. [DOI via Crossref]    [Pubmed]   
6. Sozio P, Cerasa LS, Laserra S, Cacciatore I, Cornacchia C, Di Filippo ES, et al. Memantine-sulfur containing antioxidant conjugates as potential prodrugs to improve the treatment of Alzheimer's disease. Eur J Pharm Sci. 2013; 49: 187-198. [DOI via Crossref]    [Pubmed]   
7. Afoulous S, Ferhout H, Raoelison EG, Valentin A, Moukarzel B, et al. Chemical composition and anticancer, antiinflammatory, antioxidant and antimalarial activities of leaves essential oil of Cedrelopsis grevei. Food Chem Toxicol. 2013; 56: 352-362. [DOI via Crossref]    [Pubmed]   
8. Quintans Jde S, Soares BM, Ferraz RP, Oliveira AC, da Silva TB, et al. Chemical constituents and anticancer effects of the essential oil from leaves of Xylopia laevigata. Planta Med. 2013; 79: 123-130. [DOI via Crossref]    [Pubmed]   
9. Chavan MJ, Wakte PS, Shinde DB. Analgesic and anti-inflammatory activities of the sesquiterpene fraction from Annona reticulata L. bark. Nat Prod Res. 2012; 26: 1515-1518. [DOI via Crossref]    [Pubmed]   
10. Nishida R, Shelly TE, Whittier TS, Kaneshiro KY. α-Copaene, a potential rendezvous cue for the mediterranean fruit fly, Ceratitis capitata? J Chem Ecol. 2000; 26: 87-100.
11. Vinholes J, Rudnitskaya A, Gonçalves P, Martel F, Coimbra MA, Rocha SM. Hepatoprotection of sesquiterpenoids: A quantitative structure-activity relationship (QSAR) approach. Food Chem. 2013; 146: 78-84. [DOI via Crossref]    [Pubmed]   
12. Veiga Junior VF, Rosas EC, Carvalho MV, Henriques MG, Pinto AC. Chemical composition and anti-inflammatory activity of copaiba oils from Copaifera cearensis Huber ex Ducke, Copaifera reticulata Ducke and Copaifera multijuga Hayne--a comparative study. J Ethnopharmacol. 2007; 112: 248-254. [DOI via Crossref]    [Pubmed]   
13. Togar B. The cytological, biochemical and genetic effects of selected sesquiterpenes on healthy neuron and N2a neuroblastoma cell cultures. PhD's thesis, Atatürk University, Gradute School of Natural and Applied Sciences, Department of Biology, Erzurum, Turkey. 2013.
14. Türkez H, Çelik K, Toðar B. Effects of copaene, a tricyclic sesquiterpene, on human lymphocytes cells in vitro. Cytotechnology. 2013; [DOI via Crossref]    [DOI via Crossref]   
15. Daikhin Y, Yudkoff M. Compartmentation of brain glutamate metabolism in neurons and glia. J Nutr. 2000; 130: 1026-1031.
16. Erel O. A novel automated direct measurement method for TAC using a new generation, more stable ABTS radical cation. Clin Biochem 2004; 37: 277-285. [DOI via Crossref]    [Pubmed]   
17. Erel O. A new automated colorimetric method for measuring total oxidant status. Clin Biochem., 2005; 38: 1103-1111.
18. Abdel-Wahab BA, Metwally ME. Ginkgo biloba enhances the anticonvulsant and neuroprotective effects of sodium valproate against kainic acid-induced seizures in mice. J Pharmacol Toxicol. 2011; 6:679-690. [DOI via Crossref]   
19. Miller R, James-Kracke M, Sun G, Sun A Oxidative and inflammatory pathways in Parkinson's disease. Neurochem Res. 2009; 34: 55-65. [DOI via Crossref]    [Pubmed]   
20. Cannon JR, Greenamyre JT. The role of environmental exposures in neurodegeneration and neurodegenerative diseases. Toxicol Sci. 2011; 124: 225-250. [DOI via Crossref]    [Pubmed]    [PMC Free Fulltext]   
21. McCarthy JJ, Saith S, Linnertz C, Burke JR, Hulette CM, Welsh-Bohmer KA, et al. The Alzheimer's associated 5' region of the SORL1 gene cis regulates SORL1 transcripts expression. Neurobiol Aging. 2012; 33: 1485.e1-8.
22. Chen X, Zhang Q, Cheng Q, Ding F. Protective effect of salidroside against H2O2-induced cell apoptosis in primary culture of rat hippocampal neurons. Mol Cell Biochem. 2009; 32: 85-93. [DOI via Crossref]    [Pubmed]   
23. Avalos Funez A, Isabel Haza A, Mateo D, Morales P. In vitro evaluation of silver nanoparticles on human tumoral and normal cells. Toxicol Mech Method. 2013; 23: 153-160. [DOI via Crossref]    [Pubmed]   
24. Tang LL, Wang R, Tang XC. Huperzine A protects SHSY5Y neuroblastoma cells against oxidative stress damage via nerve growth factor production. Eur J Pharmacol. 2005; 519: 9-15. [DOI via Crossref]    [Pubmed]   
25. Gan LS, Zheng YL, Mo JX, Liu X, Li XH, Zhou CX. Sesquiterpene lactones from the root tubers of Lindera aggregata. J Nat Prod. 2009; 72: 1497-1501. [DOI via Crossref]    [Pubmed]   
26. Turkez H, Sozio P, Geyikoglu F, Tatar A, Hacimuftuoglu A, Di Stefano A Neuroprotective effects of farnesene against hydrogen peroxide-induced neurotoxicity in vitro. Cell Mol Neurobiol. 2013; [DOI via Crossref]    [DOI via Crossref]   
27. Kusano C, Ferrari B. Total Antioxidant Capacity: a biomarker in biomedical and nutritional studies. J Cell Mol Biol. 2008; 7: 1-15.
28. Hou RC, Huang HM, Tzen JT, Jeng KC. Protective effects of sesamin and sesamolin on hypoxic neuronal and PC12 cells. J Neurosci Res. 2003; 74: 123-133. [DOI via Crossref]    [Pubmed]   
29. Shin HT, Chung SH, Lee JS, Kim SS, Shin HD, Jang MH, et al. Protective effect of shenqi-wan against H2O2-induced apoptosis in hippocampal neuronal cells. Am J Chin Med. 2003; 31: 675-686. [DOI via Crossref]    [Pubmed]   
30. Jiang B, Liu JH, Bao Y, An LJ. Hydrogen peroxide-induced apoptosis in PC12 cells and the protective effect of puerarin. Cell Biol Int. 2003; 27: 1025-1031. [DOI via Crossref]    [Pubmed]   
31. Iizuka Y, Hong S, Kim CY, Yang WI, Lee JE, Seong GJ. Protective mechanism of agmatine pre-treatment on RGC-5 cells injured by oxidative stress. Braz J Med Biol Res. 2010; 43: 356-358. [DOI via Crossref]    [Pubmed]   
32. Zhang Q, Huang WD, Lv XY, Yang YM. Puerarin protects differentiated PC12 cells from H2O2-induced apoptosis through the PI3K/Akt signalling pathway. Cell Biol Int. 2012; 36: 419-426. [DOI via Crossref]    [Pubmed]   
33. Vlasova IA, Zakharova IO, Sokolova TV, Avrova NF. Metabolic effects of ganglioside GM1 on PC12 cells at oxidative stress depend on modulation of activity of tyrosine kinase of trk receptor. Zh Evol Biokhim Fiziol. 2013; 49: 15-23.
34. Kikuchi Y, Yasuhara T, Agari T, Kondo A, Kuramoto S, Kameda M, et al. Urinary 8-OHdG elevations in a partial lesion rat model of Parkinson's disease correlate with behavioral symptoms and nigrostriatal dopaminergic depletion. J Cell Physiol. 2011; 226: 1390-1398. [DOI via Crossref]    [Pubmed]   

This Article Cited By the following articles

Plants-Derived Neuroprotective Agents: Cutting the Cycle of Cell Death through Multiple Mechanisms
Evidence-Based Complementary and Alternative Medicine 2017; 2017(): 1.

1
 
Cytotoxic and cytogenetic effects of α-copaene on rat neuron and N2a neuroblastoma cell lines
Biologia 2014; 69(7): 936.

2
 

How to Cite this Article
Pubmed Style

Turkez H, Togar B, Tatar A. Tricyclic sesquiterpene copaene prevents H2O2-induced neurotoxicity. J Complement Med Res (Formerly J Intercult Ethnopharmacol). 2014; 3(1): 21-28. doi:10.5455/jice.20131229104710


Web Style

Turkez H, Togar B, Tatar A. Tricyclic sesquiterpene copaene prevents H2O2-induced neurotoxicity. http://www.jicep.com/?mno=47819 [Access: February 22, 2018]. doi:10.5455/jice.20131229104710


AMA (American Medical Association) Style

Turkez H, Togar B, Tatar A. Tricyclic sesquiterpene copaene prevents H2O2-induced neurotoxicity. J Complement Med Res (Formerly J Intercult Ethnopharmacol). 2014; 3(1): 21-28. doi:10.5455/jice.20131229104710



Vancouver/ICMJE Style

Turkez H, Togar B, Tatar A. Tricyclic sesquiterpene copaene prevents H2O2-induced neurotoxicity. J Complement Med Res (Formerly J Intercult Ethnopharmacol). (2014), [cited February 22, 2018]; 3(1): 21-28. doi:10.5455/jice.20131229104710



Harvard Style

Turkez, H., Togar, B. & Tatar, A. (2014) Tricyclic sesquiterpene copaene prevents H2O2-induced neurotoxicity. J Complement Med Res (Formerly J Intercult Ethnopharmacol), 3 (1), 21-28. doi:10.5455/jice.20131229104710



Turabian Style

Turkez, Hasan, Basak Togar, and Abdulgani Tatar. 2014. Tricyclic sesquiterpene copaene prevents H2O2-induced neurotoxicity. Journal of Complementary Medicine Research (Formerly known as Journal of Intercultural Ethnopharmacology (2012-2017)), 3 (1), 21-28. doi:10.5455/jice.20131229104710



Chicago Style

Turkez, Hasan, Basak Togar, and Abdulgani Tatar. "Tricyclic sesquiterpene copaene prevents H2O2-induced neurotoxicity." Journal of Complementary Medicine Research (Formerly known as Journal of Intercultural Ethnopharmacology (2012-2017)) 3 (2014), 21-28. doi:10.5455/jice.20131229104710



MLA (The Modern Language Association) Style

Turkez, Hasan, Basak Togar, and Abdulgani Tatar. "Tricyclic sesquiterpene copaene prevents H2O2-induced neurotoxicity." Journal of Complementary Medicine Research (Formerly known as Journal of Intercultural Ethnopharmacology (2012-2017)) 3.1 (2014), 21-28. Print. doi:10.5455/jice.20131229104710



APA (American Psychological Association) Style

Turkez, H., Togar, B. & Tatar, A. (2014) Tricyclic sesquiterpene copaene prevents H2O2-induced neurotoxicity. Journal of Complementary Medicine Research (Formerly known as Journal of Intercultural Ethnopharmacology (2012-2017)), 3 (1), 21-28. doi:10.5455/jice.20131229104710





Most Viewed Articles
  • Role of Cyperus rotundus oil in decreasing hair growth
    GHADA FAROUK ABD EL-KAREAM
    J Complement Med Res (Formerly J Intercult Ethnopharmacol). 2012; 1(2): 111-118
    » Abstract & References » doi: 10.5455/jice.20120626100304

  • Ethno-dentistry: popular medicinal plants used for dental diseases in India
    Amit Bhardwaj, Shalu Verma Bhardwaj
    J Complement Med Res (Formerly J Intercult Ethnopharmacol). 2012; 1(1): 62-65
    » Abstract & References » doi: 10.5455/jice.20120322035152

  • Studies with Myrtus communis L.: Anticancer Properties
    Recai Ogur
    J Complement Med Res (Formerly J Intercult Ethnopharmacol). 2014; 3(4): 135-137
    » Abstract & References » doi: 10.5455/jice.20140803044831

  • Total phenolic, flavonoids and tannin contents in different extracts of Artemisia absinthium
    Rajeev Singh, Pawan Kumar Verma, Gagandeep Singh
    J Complement Med Res (Formerly J Intercult Ethnopharmacol). 2012; 1(2): 101-104
    » Abstract & References » doi: 10.5455/jice.20120525014326

  • Antimicrobial and phytochemical evaluation of the leaf, stem bark and root extracts of Cyathula prostrata (L) Blume against some human pathogens
    Gideon Ikechukwu Ogu, Williams Omotola Tanimowo, Perpetua Ugonna Nwachukwu, Bright Esegbuyota Igere
    J Complement Med Res (Formerly J Intercult Ethnopharmacol). 2012; 1(1): 35-43
    » Abstract & References » doi: 10.5455/jice.20120407085952

  • Herbal Antibacterials: A Review
    Chirag Modi, Shailesh Mody, Hitesh Patel, Ghanshyam Dudhatra, Avinash Kumar, Madhavi Awale
    J Complement Med Res (Formerly J Intercult Ethnopharmacol). 2012; 1(1): 52-61
    » Abstract & References » doi: 10.5455/jice.20120207113423

  • Preliminary phytochemical screening and evaluation of antibacterial activity of Dichrocepala integrifolia (L.f) O. kuntze
    Tesfaye Mohammed, Chalachew Teshale
    J Complement Med Res (Formerly J Intercult Ethnopharmacol). 2012; 1(1): 30-34
    » Abstract & References » doi: 10.5455/jice.20120401054654

  • Medicinal plants with potential anti-arthritic activity:
    Manjusha Choudhary, Vipin Kumar, Hitesh Malhotra, Surender Singh
    J Complement Med Res (Formerly J Intercult Ethnopharmacol). 2015; 4(2): 147-179
    » Abstract & References » doi: 10.5455/jice.20150313021918

  • Phytochemical and antibacterial activity of the extracts of Fagara zanthoxyloides on selected cariogenic and enteric bacterial isolates
    Martins Ajibade Adefisoye, Kwashie AjibadeAko-Nai, Mary Adejumoke Bisi-Johnson
    J Complement Med Res (Formerly J Intercult Ethnopharmacol). 2012; 1(1): 1-6
    » Abstract & References » doi: 10.5455/jice.55-1333123486

  • Effect of Momordica charantia (bitter melon) on serum glucose level and various protein parameters in acetaminophen intoxicated rabbits
    Kanwal Zahra, Muhammad Imran Sohail, Muhammad Anwar Malik
    J Complement Med Res (Formerly J Intercult Ethnopharmacol). 2012; 1(1): 7-12
    » Abstract & References » doi: 10.5455/jice.20120403013109

  • Most Downloaded
  • Studies with Myrtus communis L.: Anticancer Properties
    Recai Ogur
    J Complement Med Res (Formerly J Intercult Ethnopharmacol). 2014; 3(4): 135-137
    » Abstract & References » doi: 10.5455/jice.20140803044831

  • Total phenolic, flavonoids and tannin contents in different extracts of Artemisia absinthium
    Rajeev Singh, Pawan Kumar Verma, Gagandeep Singh
    J Complement Med Res (Formerly J Intercult Ethnopharmacol). 2012; 1(2): 101-104
    » Abstract & References » doi: 10.5455/jice.20120525014326

  • Herbal Antibacterials: A Review
    Chirag Modi, Shailesh Mody, Hitesh Patel, Ghanshyam Dudhatra, Avinash Kumar, Madhavi Awale
    J Complement Med Res (Formerly J Intercult Ethnopharmacol). 2012; 1(1): 52-61
    » Abstract & References » doi: 10.5455/jice.20120207113423

  • Ethno-dentistry: popular medicinal plants used for dental diseases in India
    Amit Bhardwaj, Shalu Verma Bhardwaj
    J Complement Med Res (Formerly J Intercult Ethnopharmacol). 2012; 1(1): 62-65
    » Abstract & References » doi: 10.5455/jice.20120322035152

  • Antimicrobial and phytochemical evaluation of the leaf, stem bark and root extracts of Cyathula prostrata (L) Blume against some human pathogens
    Gideon Ikechukwu Ogu, Williams Omotola Tanimowo, Perpetua Ugonna Nwachukwu, Bright Esegbuyota Igere
    J Complement Med Res (Formerly J Intercult Ethnopharmacol). 2012; 1(1): 35-43
    » Abstract & References » doi: 10.5455/jice.20120407085952

  • Role of Cyperus rotundus oil in decreasing hair growth
    GHADA FAROUK ABD EL-KAREAM
    J Complement Med Res (Formerly J Intercult Ethnopharmacol). 2012; 1(2): 111-118
    » Abstract & References » doi: 10.5455/jice.20120626100304

  • A review on ethnopharmacological potential of Aloe vera L.
    Md. Shamim Hossain, A.N.M Mamun-Or-Rashid, Nayeem Md. Towfique, Monokesh Kumer Sen
    J Complement Med Res (Formerly J Intercult Ethnopharmacol). 2013; 2(2): 113-120
    » Abstract & References » doi: 10.5455/jice.20130612035300

  • Use of Aqueous Extract of Corn Silk in the Treatment of Urinary Tract Infection
    Ahmed Salih Sahib, Imad Hashim Mohammed, Saba Jasim Hamdan
    J Complement Med Res (Formerly J Intercult Ethnopharmacol). 2012; 1(2): 93-96
    » Abstract & References » doi: 10.5455/jice.20120525123150

  • Preliminary phytochemical screening and evaluation of antibacterial activity of Dichrocepala integrifolia (L.f) O. kuntze
    Tesfaye Mohammed, Chalachew Teshale
    J Complement Med Res (Formerly J Intercult Ethnopharmacol). 2012; 1(1): 30-34
    » Abstract & References » doi: 10.5455/jice.20120401054654

  • Phytochemical and antibacterial activity of the extracts of Fagara zanthoxyloides on selected cariogenic and enteric bacterial isolates
    Martins Ajibade Adefisoye, Kwashie AjibadeAko-Nai, Mary Adejumoke Bisi-Johnson
    J Complement Med Res (Formerly J Intercult Ethnopharmacol). 2012; 1(1): 1-6
    » Abstract & References » doi: 10.5455/jice.55-1333123486

  • Most Cited Articles
  • Total phenolic, flavonoids and tannin contents in different extracts of Artemisia absinthium
    Rajeev Singh, Pawan Kumar Verma, Gagandeep Singh
    J Complement Med Res (Formerly J Intercult Ethnopharmacol). 2012; 1(2): 101-104
    » Abstract & References » doi: 10.5455/jice.20120525014326
    Cited : 28 times [Click to see citing articles]

  • Antimicrobial and phytochemical evaluation of the leaf, stem bark and root extracts of Cyathula prostrata (L) Blume against some human pathogens
    Gideon Ikechukwu Ogu, Williams Omotola Tanimowo, Perpetua Ugonna Nwachukwu, Bright Esegbuyota Igere
    J Complement Med Res (Formerly J Intercult Ethnopharmacol). 2012; 1(1): 35-43
    » Abstract & References » doi: 10.5455/jice.20120407085952
    Cited : 11 times [Click to see citing articles]

  • Some pharmacological effects of cinnamon and ginger herbs in obese diabetic rats
    Mostafa Abbas Shalaby, Hamed Yahya Saifan
    J Complement Med Res (Formerly J Intercult Ethnopharmacol). 2014; 3(4): 144-149
    » Abstract & References » doi: 10.5455/jice.20140818050741
    Cited : 11 times [Click to see citing articles]

  • Guava leaves polyphenolics-rich extract inhibits vital enzymes implicated in gout and hypertension in vitro
    Emmanuel Anyachukwu Irondi, Samson Olalekan Agboola, Ganiyu Oboh, Aline Augusti Boligon, Margareth Linde Athayde, Francis O. Shode
    J Complement Med Res (Formerly J Intercult Ethnopharmacol). 2016; 5(2): 122-130
    » Abstract & References » doi: 10.5455/jice.20160321115402
    Cited : 11 times [Click to see citing articles]

  • Analgesic, Anti-inflammatory and Antihyperlipidemic Activities of Commiphora molmol Extract (Myrrh)
    Mostafa Abbas Shalaby, Ashraf Abd-Elkhalik Hammouda
    J Complement Med Res (Formerly J Intercult Ethnopharmacol). 2014; 3(2): 56-62
    » Abstract & References » doi: 10.5455/jice.20140130015014
    Cited : 9 times [Click to see citing articles]

  • Potential antimalarials from African natural products: a review
    Bashir Lawal, Oluwatosin Kudirat Shittu, Adamu Yusuf Kabiru, Ali Audu Jigam, Maimuna Bello Umar, Eustace Bonghan Berinyuy, Blessing Uchenna Alozieuwa
    J Complement Med Res (Formerly J Intercult Ethnopharmacol). 2015; 4(4): 318-343
    » Abstract & References » doi: 10.5455/jice.20150928102856
    Cited : 8 times [Click to see citing articles]

  • Herbal medicine use among Turkish patients with chronic diseases
    Mnevver Tulunay, Cenk Aypak, Hlya Yklkan, Sleyman Grpeliolu
    J Complement Med Res (Formerly J Intercult Ethnopharmacol). 2015; 4(3): 217-220
    » Abstract & References » doi: 10.5455/jice.20150623090040
    Cited : 7 times [Click to see citing articles]

  • Is there a role for homeopathy in breast cancer surgery? A first randomized clinical trial on treatment with Arnica montana to reduce postoperative seroma and bleeding in patients undergoing total mastectomy
    Luca Sorrentino, Salvatore Piraneo, Eliana Riggio, Silvia Basilic, Alessandra Sartani, Daniela Bossi, Fabio Corsi
    J Complement Med Res (Formerly J Intercult Ethnopharmacol). 2017; 6(1): 1-8
    » Abstract » doi: 10.5455/jice.20161229055245
    Cited : 6 times [Click to see citing articles]

  • Study of antinociceptive and anti-inflammatory activities of certain Iranian medicinal plants
    Fariba Sharififar, Payam Khazaeli, Narguess Alli, Elham Talebian, Raheleh Zarehshahi, Shivasadat Amiri
    J Complement Med Res (Formerly J Intercult Ethnopharmacol). 2012; 1(1): 19-24
    » Abstract & References » doi: 10.5455/jice.20120227104636
    Cited : 5 times [Click to see citing articles]

  • Preliminary phytochemical screening and evaluation of antibacterial activity of Dichrocepala integrifolia (L.f) O. kuntze
    Tesfaye Mohammed, Chalachew Teshale
    J Complement Med Res (Formerly J Intercult Ethnopharmacol). 2012; 1(1): 30-34
    » Abstract & References » doi: 10.5455/jice.20120401054654
    Cited : 5 times [Click to see citing articles]